Marcus bauser medicinal chemistry vii, business group pharma, bayer ag, 42096 wuppertal, germany preparative hplc when coupledhyphenated with other analytical techniques. Ymc america offers a variety of different lc columns, components, and separation materials. Ymc chiral art is available in 3 m and 5 m analytical and preparative hplc columns and as preparative bulk media with 10 m or 20 m. Discovery semiprep and preparative hplc columns discovery is a suite of hplc columns featuring functionalized reversedphases based on unique combinations of polar and hydrophobic retention mechanisms. Initial analytical hplc runs and the final preparative requirements have two key differences. Ymc europe gmbh schottmannshof 19 46539 dinslaken phone. Unlike traditional column packing methods, the axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative hplcsfc columns. The solvents for preparative chromatography were deionized water solvent a and 96% ethanol in water solvent b. Column stability is improved by increasing the packed bed density. Preparative and semipreparative columns capital hplc. Ymc actus series columns show high durability under high flow rate or steep gradient conditions and desirable for milligram scale preparative hplc of various compounds. Ymc is known worldwide for the variety, versatility and quality of its hplc packings. Mar 28, 2014 preparative highperformance liquid chromatographymass spectrometry for the high throughput purification of combinatorial libraries. The scaleup calculator is here to help you with the scale up of the chromatographic parameters such as flow rate, the amount of packing material, etc.
Common techniques used for this purpose are various forms of axial or radial compression. Discovery semiprep and preparative hplc columns semiprep. Hplc columns perkin elmer 02580191 hplc column manufacturer. An interchim prep column will operate at lower pressure which translates into longer column life. Furthermore, uhplc columns and preparative columns are also available. Cosmosil application has more than 7,000 applications using cosmosil columns. Ymc triart series are nextgeneration organic hybrid silica based columns, emphasizing versatility. I want to do preparative hplc for fermented sample. What are preparative, semipreparative and analytical hplc. Im analysing the degradation of pharmaceuticals using hplc with c18 columns. Axia packed preparative columns involve a single axial compression step unlike conventional packed preparative columns. Ascentis c18 preparative columns 510 55840u 510 105841u 510 155842u 510 255843u 5 21.
Ymcs preparative systems are applicable to various preparative scales from small scale to large scale. Ymc use a highpressure slurry technique to pack all preparative columns in option 1,200 or 2,000 psi pressure rated hardware. Global distributor of uhplc columns and highperformance lc supplies. If columns are affected by undesired contaminants or clogged inlet frits which cause back pressure increases, flush the column with thf in the opposite flow direction. Ymcactus series are semipreparative hplc columns that have excellent column durability and efficiency by applying axial compression technology. The main features are superior durability, peak shape across all kind of compounds and reproducibility. The recommended ph range for using ymc chiral polysaccharide derivatives columns is 3. Versatile hybrid silica based uhplc columns the selectivity. Ymcpack preparative columns, packed with ymcgels, are available in a broad variety of column sizes to accommodate virtually any preparative separation. The columns were packed with reprosil 100 c18 with 15 m particle size. Unlike traditional column packing methods, the axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative hplc sfc columns. The ideal column bed density is custom calculated and automated for each specific media and column size. Preparative highperformance liquid chromatographymass spectrometry for the high throughput purification of combinatorial libraries.
Cosmosil application provides you with sample analysis conditions with widely used ods columns and other specialty columns. Ymc america makes it simple to buy ultra high performance liquid chromatography products and high performance liquid chromatography products online thanks to our responsive customer support, wealth of informational resources, and wide variety of lc columns, accessories and other supplies. In expanding the distribution of ymc columns globally, waters maintains exclusive distribution rights. Our chiral columns are ideal for chromatography techniques such as supercritical fluid chromatography sfc. Excellent axia prepacked preparative hplc sfc columns. The product portfolio ranges from classical analytical over chiral to semi preparative hplc columns. Our chiral columns are ideal for chromatography techniques such as supercritical fluid chromatography sfc, hplc, prep scale lc, and simulated moving bed smb.
Shimpack gis series columns are packed with high purity silica gel and are your ideal generic hplc columns. Silica ods columns with octadecylsilylated silica gel are the most common in this mode. Ymc s preparative systems are applicable to various preparative scales from small scale to large scale. The shimpack gis series not only reduces your cost of solvents, but relieves your system load as well. Semi preparative system 20ar type a system using lc20ar supports columns from analytical columns to 20 mm i. Ymc were first to set a milestone with the legendary ymcpack odsaq, a c18 phase stable in aqueous conditions, for the reversed phase.
Ymc is one of the most established manufacturers of hplc columns. Waters corporation announces worldwide distribution of the ymc hplc analytical and preparative column lines, a highly respected series of products that waters has distributed since 1997. Available in over 40 unique achiral and chiral selectivities, axia prep lc columns offer. Furthermore every column of ymc is individually tested to provide peak symmetry and general performance as well as the high quality standards of ymc pro. Ymc provides various products, including hplc columns, ion exchange chromatography columns packing materials for purification of biopharmaceuticals, and chiral chromatography columns packing materials, which can separate a wide range of compounds. Ymcactus series columns show high durability under high flow rate or steep gradient conditions and desirable for milligram scale preparative hplc of various compounds. Ymctriart series are nextgeneration organic hybrid silica based columns, emphasizing versatility. Ymctriart c18, c8 and hilicdiol in their particle sizes of 1. The presence of fines in the column will gradually migrate to the outlet frit where they are trapped and cause pressure build up. Ymcspecialty columns capital hplc limited hplc columns. The ultimate prepacked preparative column for hplc and sfc. Further, ymcpack odsaq was the first so called aqcolumn, which can be used under 100% aqueous conditions. Ymc use a highpressure slurry technique to pack all preparative columns in. Ymctriart columns for sfc the analytical scientist.
Ymc is one of the very few global players in the market that meet the challenging demands in preparative scale chromatography. Due to their high quality and reproducibility, ymcpack ods columns are successfully used for the validation of analytical hplc methods and for long term reproducible preparative hplc processes. On the other hand, ymctriart c18 exrs has been designed to provide con. Highly pure cannabidiol cbd by twincolumn chromatography. Ymcs bestselling, worldrenowned ymcpack odsseries is widely known for its variety of selectivities for nonpolar, moderately polar and polar compounds. An attention centred originally in the analytical requirements of their clients has evolved to develop a. Computer control of the entire process ensures both proper bed density and column uniformity every time. The columns had an inner diameter of 10 mm and a bed height of 150 mm.
Daniel eer ymc column hardware availability specications. Ymc actus series are semi preparative hplc columns that have excellent column durability and efficiency by applying axial compression technology. The best suited packing material depends significantly on the characteristics of the separation conditions, which should be thoroughly considered. Ymc provides various products, including hplc columns, ion exchange chromatography columnspacking materials for purification of biopharmaceuticals, and chiral chromatography columnspacking materials, which can separate a wide range of compounds. Polysaccharide chiral hplc columns, both coated and immobilised types, are available for a wide range of applications. Setting optimal hplc experimental parameters is the one of the most important processes that requires experience and time. Highly uniform particles ensure stable mobile phase delivery and outstanding low pressure. An attention centred originally in the analytical requirements of their clients has evolved to develop a full range of packings whose.
Excellent axia prepacked preparative hplcsfc columns. Ymctriart c18 has a higher carbon load, giving it a hydrophobicity comparable to that of standard ods columns, thereby making it a versatile firstchoice column for method development. Plrps for prep the plrps prep to process media is available in a range of pore sizes for small molecule, synthetic biomolecule and macromolecule purification. Introduction of preparative systems according to purpose. Having the same selectivity across different particle sizes, smooth method transfer. Increased performance, groundbreaking lifetimes, optimized loadability, and increased reproducibility. To find similar hplc columns to test as backup columns, follow these steps. For example in reversephase high performance liquid chromatography by using 250 cm length x 4. Plrps for prep the plrps prep to process media is available in a range of pore sizes for small molecule. Uhplc to hplc analysis and even to preparative separation.
Ymc also provide total solutions for preparative chromatography, such as most suitable packing materials and columns in addition to preparative systems. Reliable and robust iex columns by ymc the analytical scientist. Practical aspects of preparative hplc in pharmaceutical and. Unifinepak c18 crestpak c18t5, c8t5, c15 finepak sil c18t5. List of analytical solutions, instruments hplc, gc, standards and reagents provided by ymc co. Semipreparative system 20ar type a system using lc20ar supports columns from analytical columns to 20 mm i. Versatile uhplchybrid columns with ymctriart challenging phstability and high temperatures are no longer a limitation to your work. Ymc analytical hplc columns in order to develop a successful hplc method, the choice of the optimal selectivity is essential to establish efficient separation conditions. The best suited packing material depends significantly on the characteristics of the separation conditions, which should be. Preparative hplc columns and bulk media from phenomenex.
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